nis-element viewer 4.50 Search Results


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Nikon confocal microscope
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
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Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
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Nikon lasers
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Lasers, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon a1rs hd confocal microscope system
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
A1rs Hd Confocal Microscope System, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SoftMax Inc pro v 7.0
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Pro V 7.0, supplied by SoftMax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millar Inc pressure–volume (pv) catheter
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Pressure–Volume (Pv) Catheter, supplied by Millar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM VisualSonics Inc vevo 3100 echocardiography system
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Vevo 3100 Echocardiography System, supplied by FUJIFILM VisualSonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Abcam alexa fluor 488 goat anti mouse igg h l
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Alexa Fluor 488 Goat Anti Mouse Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc paper n a pcl eco addgene
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
Paper N A Pcl Eco Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
STEMCELL Technologies Inc l-calc software stemcell #28600
Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto <t>microscope</t> cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.
L Calc Software Stemcell #28600, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto microscope cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.

Journal: Molecular Therapy Oncology

Article Title: CDCP1-targeting ADC outperforms standard therapies in Ras-mutant pancreatic cancer

doi: 10.1016/j.omton.2025.201024

Figure Lengend Snippet: Internalization of the 2G10 antibody in pancreatic cancer cells (A) Cells were seeded onto microscope cover glasses in a 12-well culture plates: PANC-1 (1 × 10 5 cells), AsPC-1 (2 × 10 5 cells), BxPC-3 (2 × 10 5 cells), and MIA PaCa-2 (0.5 × 10 5 cells). After blocking with a blocker for 30 min, cells were treated for 1 h at 4°C with 2G10 antibody (10 μg/mL) conjugated with the anti-mouse IgG-conjugated Alexa 488 (10 μg/mL). Lysosomes were stained with anti-LAMP-1 for 1 h, and the nuclei were counterstained with DAPI (100 nM) at room temperature. The fluorescent images were captured using a confocal microscope at 60× magnification. The experiments were repeated independently at least three times. (B) Pancreatic cancer cells were pre-incubated with CHX (75 μg/mL) and blocked with Fc blocker for 10 min to inhibit the Fc receptor-mediated internalization. The cells were then incubated in the presence or absence of 2G10 antibody (100 ng/mL) at 4°C or 37°C for 2 h and followed by flow cytometry analysis. The fluorescent signal of the 2G10 antibody/CDCP1 complex on the cell surface decreased after incubation at 37°C.

Article Snippet: Images were captured with a confocal microscope (A1R HD25 N-SIM S, Nikon, Tokyo, Japan) and obtained using NIS-Elements Viewer 4.50 software (Nikon).

Techniques: Microscopy, Blocking Assay, Staining, Incubation, Flow Cytometry